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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
24/01/2006 |
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Data da última atualização: |
25/02/2013 |
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Autoria: |
CALIGIORNE, R. B.; RESENDE, M. A.; PAIVA, E.; AZEVEDO, V. |
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Afiliação: |
Embrapa Milho e Sorgo. |
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Título: |
Use of RAPD (random amplified polymorphic DNA) to analyse genetic diversity of dematiaceous fungal pathogens. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Canadian Journal of Microbiology, Ottawa, v. 45, n. 5, p. 408-412 , 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
A total of 13 strains of fungi, the aetiological agents of chromoblastomycosis and phaeohyphomycosis (including Rhinocladiella sp., R. aquaspersa, Fonsecaea pedrosoi, F. compacta, Phialophora verrucosa, Cladosporium carrionii, Cladophialophora bantiana and Exophiala dermatitis [Wangiella dermatitidis]) were obtained from different geographical origins. These strains were genotypically compared by random amplified polymorphic DNA (RAPD). The data generated showed a high degree of polymorphism between distinct species and a low polymorphism between strains of the same species. The results generated by these tests were subjected to a numerical taxonomy analysis, using the unweighted pair-group method. A phenogram was constructed for the set of strains studied. Based on its structure, it is concluded that genotypical data provided enough information to use the unweighted pair-group method to cluster the strains in accordance to their respective species. The phenogram grouped in a single branch the strains of Fonsecaea pedrosoi and F. compacta species, indicating a great similarity between these fungi, and suggesting that the classification as distinct species may not be appropriate for these species of the genus Fonsecaea.. |
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Palavras-Chave: |
Genetic diversity; Identification; Phaeohyphomycosis; Random amplified polymorphic DNA; Techniques. |
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Thesagro: |
DNA. |
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Thesaurus NAL: |
classification; polymorphism; taxonomy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02037naa a2200265 a 4500
001 1489143
005 2013-02-25
008 1999 bl uuuu u00u1 u #d
100 1 $aCALIGIORNE, R. B.
245 $aUse of RAPD (random amplified polymorphic DNA) to analyse genetic diversity of dematiaceous fungal pathogens.$h[electronic resource]
260 $c1999
520 $aA total of 13 strains of fungi, the aetiological agents of chromoblastomycosis and phaeohyphomycosis (including Rhinocladiella sp., R. aquaspersa, Fonsecaea pedrosoi, F. compacta, Phialophora verrucosa, Cladosporium carrionii, Cladophialophora bantiana and Exophiala dermatitis [Wangiella dermatitidis]) were obtained from different geographical origins. These strains were genotypically compared by random amplified polymorphic DNA (RAPD). The data generated showed a high degree of polymorphism between distinct species and a low polymorphism between strains of the same species. The results generated by these tests were subjected to a numerical taxonomy analysis, using the unweighted pair-group method. A phenogram was constructed for the set of strains studied. Based on its structure, it is concluded that genotypical data provided enough information to use the unweighted pair-group method to cluster the strains in accordance to their respective species. The phenogram grouped in a single branch the strains of Fonsecaea pedrosoi and F. compacta species, indicating a great similarity between these fungi, and suggesting that the classification as distinct species may not be appropriate for these species of the genus Fonsecaea..
650 $aclassification
650 $apolymorphism
650 $ataxonomy
650 $aDNA
653 $aGenetic diversity
653 $aIdentification
653 $aPhaeohyphomycosis
653 $aRandom amplified polymorphic DNA
653 $aTechniques
700 1 $aRESENDE, M. A.
700 1 $aPAIVA, E.
700 1 $aAZEVEDO, V.
773 $tCanadian Journal of Microbiology, Ottawa$gv. 45, n. 5, p. 408-412 , 1999.
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